Production of milk-clotting enzyme by Bacillus subtilis B1 from wheat bran
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چکیده
منابع مشابه
Production of Milk Clotting Enzyme from Aspergillus oryzae under Solid-State Fermentation using Mixture of Wheat Bran and Rice Bran
Microbial rennet-like milk-clotting enzymes are aspartic proteinases that catalyze milk coagulation, substituting calf rennet. Crude enzymatic extract produced by the Aspergillus oryzae NCIM 1032, on solid state fermentation (SSF) using mixture of wheat bran and rice bran (7:3), exhibited high milkclotting activity (MCA) and low proteolytic activity (PA) after 120 h of fermentation. Highest mil...
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Suitable medium for production of milk clotting enzyme (MCE) by Bacillus subtilis (natto) Takahashi in submerged liquid-state fermentation was screened, the nutrient factors affecting MCE production was optimized by response surface methodology. The MCE production by B. subtilis (natto) Takahashi was increased significantly by 428% in the optimal medium developed. The MCE was filtered and conce...
متن کاملPurification and Characterization of Milk Clotting Enzyme Produced by Rhizomucor Rmiehei
Milk clotting enzyme (M CE) produced by: Rhizomucor miehei was purified and characterized.The enzyme was purified 220.29-fold with specific activity about 14444.2 U/mg protein byultrafiltration, ammonium sulfate fractionation, Sephacryl S-300 chromatography. The maximumenzyme activity was at 65°C.The milk clotting activity was decreased steadily as pH is increased and indicated maximumactivity ...
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Objective: B.subtilis and B.licheniformis have been reported as one of the potential protease producer. Hence, an attempt has been made to optimize and compare the enzyme production by entrapment and fermentor conditions. Methods: Growth profile of both the strains has been studied. Optimization of culture conditions with respect to pH, temperature, agitation, carbon, nitrogen sources and metal...
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We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2011
ISSN: 1684-5315
DOI: 10.5897/ajb10.1647